Multiple comparison correction (MCC)#
In this notebook, we’ll continue with the topic of multiple comparison correction (MCC).
What you’ll learn: after this lab, you’ll …
know the relative advantages and disadvantages of different MCC techniques
Estimated time needed to complete: 1 hour
Credits: This notebook is based on a blog by Matthew Brett and a previous Matlab-based lab by H. Steven Scholte.
Why do we need MCC?#
Univariate analyses of fMRI data essentially test hypotheses about your data (operationalized as contrasts between your \(\hat{\beta}\) estimates) for each voxel separately. So, in practice, given that the MNI (2 mm) standard template brain contains about 260,000 voxels, you’re conducting 260,000 different statistical tests! The obvious problem, here, is that some tests might turn out significant, while they in fact do not contain any (task-related) activity: the result is driven just by chance.
As a researcher, you should strive to “filter out” the results which are driven by noise (false positives) and keep the results which are actually driven by the true effect (true positives) as much as possible. It turns out that the more tests you do, the larger the chance is that you will find one or more false positives. To deal with this, researchers often employ techniques for multiple comparison correction (MCC): correcting for the inflated chance of false positives when you have multiple tests (comparisons).
In this tutorial, we will walk you through an example (simulated) dataset on which different MCC techniques are employed. We’ll focus on how these different techniques influence the chance for finding false positives.
The example#
We’ll work with the (simulated) group-level results of a hypothetical fMRI experiment. Suppose that the subjects in our hypothetical experiment were shown pictures of cats in the scanner, because we (the experimenters) were interested in which voxels would (de)activate significantly in reponse to these cat pictures (i.e. a contrast of the cat-picture-condition against baseline).
An example of an image shown to the subjects:
After extensive preprocessing, we fitted first-level models in which we evaluated the cat-against-baseline contrast, in which the \(t\)-statistic refers to how strongly each voxel responded to the pictures of cats. After doing a proper group-level analysis, we now have a group-level \(t\)-statistic map, reflecting whether voxels on average (de)activated in response to the pictures of cats.
ToThink (0.5 point): On average, what group-level $t$-statistic would you (approximately) expect to find if, in fact, there would be no voxel which reliably (de)activated in response to the cat-pictures?
YOUR ANSWER HERE
The data#
Usually, your whole-brain group-level results are 3D \(z\)- or \(t\)-statictic maps of the size of a standard brain (usually the MNI 2mm template, which has about 260,000 voxels). Plotting in 3D, however, is incredibly cumbersome, so for the sake of the example, we’ll assume that our group-level results are represented as a 2D \(z\)-statistic map, with dimensions \(200 \times 200\). So, we’ll pretend we analyzed the results based on a 2D brain with \(200 \times 200\) “voxels”.
Because we work with simulated data, we can actually specify the “true effect”. In reality, we never know this of course! We are going to assume that there is a small “blob” of voxels in the middle of our “brain” that activates reliably to pictures of cats (with a \(z\)-value of 5.5). This blob is therefore the true effect in our simulation.
Let’s first simulate the data.
import numpy as np
# You don't have to understand how this simulation works exactly
k = 200 # number of vox in each dimension
signal = np.zeros((k, k))
r = 10 # middle of the image
a, b = k // 2, k // 2 # width and height of the circle
y, x = np.ogrid[-a:k-a, -b:k-b]
mask = x * x + y * y <= r * r
signal[mask] = 5.5 # amplitude of effect!
print("Shape of statistic map: %s" % (signal.shape,))
Shape of statistic map: (200, 200)
Alright, now let’s plot the true effect as a 2D image. We’ll define a custom function for this, plot_sim_brain, to save us some work later.
import matplotlib.pyplot as plt
def plot_sim_brain(brain, mask=None, vmin=-7, vmax=7, cmap='seismic', title='', label='Z-value'):
""" Plots an image of a simulated 2D 'brain' with statistic values, which may be 'masked'.
Parameters
----------
brain : numpy array
A 2D numpy array with statistics
mask : numpy array (or None)
A 2D numpy array with booleans (True = do plot, False = do not plot). If None,
the 'brain' is not masked.
vmin : int/float
Minimum value of colorbar
vmax : int/float
Maximum value of colorbar
cmap : str
Name of colormap to use
title : str
Title of plot
label : str
Label for colorbar
"""
brainm = brain.copy()
if mask is not None: # threshold!
brainm[~mask] = 0
plt.figure(figsize=(8, 10))
plt.imshow(brainm, vmin=vmin, vmax=vmax, aspect='auto', cmap=cmap)
plt.axis('off')
plt.title(title, fontsize=25)
cb = plt.colorbar(orientation='horizontal', pad=0.05)
cb.set_label(label, fontsize=20)
plt.show()
plot_sim_brain(signal, title="True effect")
Now, due to the inherent spatial smoothness of fMRI, this particular manifestation of the effect is not very realistic. In particular, the sharp “edges” of the effect are unlikely to occur in real fMRI data. Therefore, to make it a little more realistic, we can spatially smooth the “true effect map”! We will use the gaussian_filter function (from scipy.ndimage) with a FWHM of 12 “voxels”.
from scipy.ndimage import gaussian_filter
fwhm = 12
# Convert FWHM to sigma
sigma = fwhm / np.sqrt(8 * np.log(2))
signal_smooth = gaussian_filter(signal, sigma=sigma)
plot_sim_brain(signal_smooth, title="True effect (smooth)")
As you’ve learned in the past weeks, the chances are very slim that you’ll find such a “crisp” (true) effect as shown above; often, you might observe significant voxels that are not driven by a true effect, but by (spurious) noise, reflecting false positives.
So, let’s make our data a little more realistic by simulating some random noise, sampled from a normal distribution with mean 0 and a standard deviation of 1. Importantly, we are also going to smooth our noise with the same gaussian filter (with FWHM = 12):
np.random.seed(2) # for reproducibility
noise = np.random.normal(0, 1, size=signal.shape)
noise = gaussian_filter(noise, sigma=sigma)
noise = noise / noise.std()
plot_sim_brain(noise, title='The noise')
Now, to complete our simulation, we’ll simply add the signal and the noise together (we’ll call this variable data).
data = signal_smooth + noise
plot_sim_brain(data, title='The data!')
The plot above now represents our simulated data, which contains both a true signal (the “blob” in the middle) and some (spatially correlated) noise. As a researcher, you aim to threshold your data in such a way that you maximize the chance of finding your true signal (true positive effects) and minimize the chance of erroneously treating noise as significant effects (false positive effects).
Uncorrected statistics maps#
In the early days of fMRI analyses, the extent of the MCC problem (more tests = more false positives) was not yet widely known. What researchers simply did was to calculate the \(p\)-values corresponding to the \(z\)-value (or \(t\)-value) maps and threshold those \(p\)-values using some fixed cutoff (“alpha value”), usually 0.05 or 0.01.
To implement this, we can convert all our \(z\)-values to \(p\)-values, compute a “mask” (i.e., an array with True and False values, indicating which “voxels” survive the threshold and which do not), and set all “voxels” outside the mask to 0.
Let’s choose a significance level (\(\alpha\)) of 0.05.
alpha = 0.05
Now, let’s convert the \(z\)-values (in the variable data) to \(p\)-values. We’ll use the stats.norm.sf function from the scipy package for this. (This is the same type of function — a “survival function” — that we used to calculate the \(p\)-values corresponding to \(t\)-values before, but this time we use it for \(z\)-values)
# This line converts the z-values to p-values
from scipy import stats
data_pvals = stats.norm.sf(data)
ToDo (1 point): Compute how many voxels are deemed to be "significant" (assuming $\alpha = 0.05$), using the variable data_pvals, in this approach in which we neglect the multiple comparison approach. Store this number (an integer) in a new variable named nsig_uncorrected.
# Implement your ToDo here
# YOUR CODE HERE
raise NotImplementedError()
''' Tests the above ToDo. '''
from niedu.tests.nii.week_6 import test_nsig_uncorrected
test_nsig_uncorrected(data_pvals, alpha, nsig_uncorrected)
We can create a “mask” by comparing our \(p\)-values to our significance level and we can give this mask to our plotting-function (plot_sim_brain), which will set all “voxels” outside the mask (i.e., those which are False in the mask) to 0.
smaller_than_alpha = data_pvals < alpha
# Note that 'smaller_than_alpha' is a 2D numpy array with booleans
plot_sim_brain(data, mask=smaller_than_alpha, title=r'Uncorrected ($p < %.4f$)' % alpha)
ToDo/ToThink (1 point): Change the value of $\alpha$ (i.e., the variable alpha) from before to 0.01. Does the resulting thresholded map look "better"? And what about 0.001? And 0.0001? Theoretically, you could try different values to see what gives the "best" results. This practice of trying out different parameters or strategies leads to another problem: can you think of what this could be? Write down your answer below.
YOUR ANSWER HERE
Bonferroni-correction#
Obviously, given that we know our “true effect”, we can see that the uncorrected results contain a lot of false positives, something that we’d like to avoid! The most obvious way to counter the MCC problem is to adjust the significance level (\(\alpha\)) by the amount of tests we’re performing. Bonferroni correction is such an approach. The way the Bonferroni method does this is by simply dividing the significance level by the amount of tests.
ToDo (1 point): Calculate the Bonferroni-adjusted significance level (and store this in a variable named bonf_alpha) and create a new mask by comparing the previously computed $p$-values against this new significance level. Then, plot the data (using plot_sim_brain) with the mask you just created.
""" Implement the ToDo here. """
# YOUR CODE HERE
raise NotImplementedError()
''' Tests the above ToDo. '''
from niedu.tests.nii.week_6 import test_bonferroni_alpha
test_bonferroni_alpha(alpha, data, bonf_alpha)
ToThink (not graded): Many argue that Bonferroni correction for whole-brain fMRI results is too strict (conservative), which is also the case in our simulation (the recovered "blob" is a lot smaller than the true effect).
This conservative nature of Bonferroni correction, for fMRI at least, is due to the violation of a crucial assumption of Bonferroni correctoin. Which assumption is this, and why does fMRI data/results likely violate this assumption?
YOUR ANSWER HERE
FDR correction#
As you’ve seen so far, uncorrected results tend to be too liberal (too many false positives) and Bonferroni-corrected results are too strict (too many false negatives). The “False Discovery Rate-correction” (FDR) technique is a method to adjust \(p\)-values in a less stringent way. Essentially, while traditional MCC methods (such as Bonferroni) try to control the chance of finding at least one false positive result amongst all your tests (i.e. controlling the “familywise error rate” method), the FDR-method tries to limit the proportion of false positives amongst all your tests which turned out significant. So, if you set your “FDR-proportion” (confusingly also referred to as “alpha”) to 0.05, then it will adjust your initial \(p\)-values such that out of all your significant results, on average 5% will be false positives.
In general, FDR-correction is more sensitive than the Bonferroni correction method (i.e. FDR has a lower type 2 error rate/it is less strict), but if you use it, you do have to accept that about 5% of your (significant) results are false positives!
Now, let’s check out what our results look like after FDR correction:
# fdr implementation from statsmodels package
from fdr import fdrcorrection
alpha_fdr = 0.05 # we use an alpha of 0.05 (5%)
# The fdrcorrection function already returns a "mask"
# Note that it doesn't accept 2D arrays, so we ravel() and then reshape() it
fdr_mask = fdrcorrection(data_pvals.ravel(), alpha=alpha_fdr)[0]
fdr_mask = fdr_mask.reshape(data.shape)
plot_sim_brain(data, mask=fdr_mask, title='FDR correction')
As you can see, the FDR-correction is way more sensitive than the Bonferroni correction (it “recovers” more of the true signal), but it still results in many false positives (but not as many as uncorrected data).
RFT-based correction#
As you’ve seen in the previous examples, it’s quite hard to pick a significance level that strikes a good balance between type 1 errors and type 2 errors, or, phrased differently, between sensitivity (with respect to discovering the true signal) and specificy (i.e. how many of our significant voxels are driven by a true effect).
Let’s go back to the results of the Bonferroni correction. We’ve seen that the results are extremely conservative (few false positives, but many false negatives, i.e. large type 2 error). The major reason for this is that the correction assumes that each test is independent, but in our simulation (and in any fMRI dataset), we know that there exists spatial correlation, meaning that our tests are not independent. In other words, if we know that a certain voxel is signficant in a certain test, it is quite likely that the voxel directly next (or above/below) to it is also significant. Therefore, spatially correlated fMRI statistic maps violate Bonferroni’s assumption of independent tests (this is also the answer to the ToThink from earlier).
As a possible solution to this problem, neuroscientists have developed a method — random field theory — that allows for multiple comparison correction (using FWER) that “corrects” for the smoothness in our data and thresholds accordingly.
Importantly, RFT-correction can either be performed at the voxel-level (testing whether the amplitude, i.e., height of the statistic of a voxel is significant, given the smoothness of the data) and at the cluster-level (testing whether the size of a cluster of voxels is significantly large, given the smoothness of the data). We’ll start with voxel-level RFT.
Voxel-level RFT#
Voxel-level RFT allows for “smoothness-adjusted” thresholding for individual voxels. It does so by assuming a particular distribution for the number of clusters (or “blobs”) one would observe given (1) a particular initial threshold and (2) the smoothness of the data, assuming there is no effect (i.e., the null hypothesis is true). This expected “number of blobs” after thresholding is known as the Euler characteristic. And for standard normal data (i.e., \(z\)-statistics), the expected Euler characteristic is computed as:
(83)#\[\begin{align}
EC = N_{resel}\ (4\ \log_{e}2)\ (2\pi)^{-\frac{2}{3}}\ z\cdot e^{-\frac{1}{2} z}
\end{align}\]
where \(R\) refers to the number of “resels” (a number that depends on the smoothness of your data, which we’ll discuss in a bit) and \(z\) refers to the \(z\)-value that you use as an initial threshold. In code, this is:
def expected_EC(z, n_resel):
""" Computes the expected Euler Characteristic for a given number of resels
and initial z-value cutoff.
Parameters
----------
z : int/float or array of int/float
Initial z-value cutoff (can be array)
n_resel : int/float
Number of "resels"
"""
return n_resel * (4 * np.log(2)) * (2 * np.pi) ** (-(2/3)) * z * np.exp(-0.5 * z ** 2)
Importantly, suppose for now that the number of resels is 1000. Then, we can get the expected number of “blobs” in our data for a given \(z\)-value threshold, let’s say \(z = 3\), as follows:
zthresh = 3
n_blobs = expected_EC(z=zthresh, n_resel=1000)
print("For a z-threshold of %i, we expect %.2f blobs in random 2D data with 100 resels." % (zthresh, n_blobs))
For a z-threshold of 3, we expect 27.14 blobs in random 2D data with 100 resels.
We can also evaluate the expected EC for a range of potential \(z\)-value thresholds (e.g., from 0-5) and plot it:
zx = np.linspace(0, 5, 100) # 100 values between 0 and 5
ecs = expected_EC(zx, n_resel=1000) # expected EC also works for multiple z-values at once
plt.figure(figsize=(10, 4))
plt.plot(zx, ecs)
plt.ylabel('Expected EC', fontsize=20)
plt.xlabel('Z-value threshold', fontsize=20)
plt.grid()
To compute the Euler characteristic, we first need to know how to estimate the number of “resels” for our data. You can think of the number of resels as the number of truly independent elements in your data (“resel” is short for “RESolution ELement”). The number of resels is usually estimated by dividing the number of voxels by the estimated size of the resel. For our simulated 2D data, the number of resels is defined as follows:
(84)#\[\begin{align}
N_{resel} = \frac{N_{X}\cdot N_{Y}}{\mathrm{size}_{resel}}
\end{align}\]
where \(N_{X}\) is the number of “voxels” in the first dimension and \(N_{Y}\) the number of “voxels” in the second dimension, and where the resel size (\(\mathrm{size}_{resel}\)) is estimated as the product of the smoothness of our data in all dimensions, measured in FWHM:
(85)#\[\begin{align}
\mathrm{size}_{resel} = \mathrm{FWHM}_{X} \cdot \mathrm{FWHM}_{Y}
\end{align}\]
So, given a particular size of our resel, \(N_{resel}\) represents how many resels there would “fit” in our data.
ToDo (1 point): Usually, the smoothness of the data has to be estimated (usually from the residuals), but in our simulation, we know the smoothness: it's the FWHM we used for our gaussian filter to smooth out data! Compute the number of resels in our simulated data and store it in a variable named n_resel.
# Implement your ToDo here
# YOUR CODE HERE
raise NotImplementedError()
''' Tests the above ToDo. '''
from niedu.tests.nii.week_6 import test_n_resel
test_n_resel(data, n_resel)
Now, another way to interpret EC values is as \(p\)-values: the chance of finding one or more “blobs” for a given \(z\)-value! This way, we can choose a particular \(z\)-value threshold that would correspond to \(p = 0.05\). We do this below:
ecs = expected_EC(zx, 278)
# find the index of the EC value closest to alpha
idx_z = np.abs(ecs - alpha).argmin()
# Index the z-values with idx_z
z_thresh = zx[idx_z]
print("The z-value threshold corresponding to p = 0.05: %.3f" % z_thresh)
The z-value threshold corresponding to p = 0.05: 4.444
ToThink (1 point): As you (should) see in the plot above, the RTF-based correction is still quite strict/conservative (i.e. misses quite some of the true effect), although arguably not as strict as Bonferroni correction. Given the way how to calculate the number of RESELS, can you think of two ways on how to improve the sensitivity of RFT-based MCC?
YOUR ANSWER HERE
Cluster-level RFT#
In all the previous MCC techniques, we have used voxel-level corrections, which resulting \(p\)-values tell us something about whether the height of a voxel’s statistic (often referred to as “amplitude”) is higher than would be expected under the null-hypothesis. Basically, because we investigated per voxel whether its value is higher than expected, we are making inferences on the level of voxels.
Another type of inference is cluster-level inference, in which you do not test the voxel amplitude, but the size of clusters. Basically, in this type of cluster-extent testing, you are investigating whether the size of the clusters you find are (significantly) larger than to be expected under the null-hypothesis (i.e., no effect).
However, as you can imagine, the null-distribution of cluster sizes (i.e. the size of “significant” clusters you’d expect by chance alone) depends strongly on the initial smoothness of your data. Again: RFT to the rescue!
Basically, RFT can also give us the \(p\)-value for clusters, given their size, by estimating the null-distribution of cluster-sizes based on the data’s smoothness. So, instead of giving us the \(p\)-value for voxels based on the height of their value and the data’s smoothness (i.e., voxel-level RFT), RFT can also do this on the cluster-level by investigating the \(p\)-value of the size of clusters. See how these two RFT-methods relate to each other? They’re doing the same thing — estimating a null-distribution given the smoothness of the data — but for different things: either for the height of the (\(z\)-)statistic value per voxel (voxel-level RFT) or for the size per cluster (cluster-level RFT).
How RFT does this is way beyond the scope of this course, but we’ll walk you through it conceptually, so that you understand the implications of this technique.
Anyway, a first step in cluster-level RFT is to determine a minimum (cutoff) value for your statistics map, which you can use to evaluate whether there are actually clusters in your data. Let’s look at an example, in which we use a minimum value of 3.0:
min_z = 3
thresh_data = (data > min_z)
plot_sim_brain(data, mask=thresh_data, title=r"Clusters after thresholding at $z$ > 3")
Now, we can use cluster-based RFT to calculate the \(p\)-value for each cluster in the above thresholded data plot. This \(p\)-value reflects the probably of this cluster-size (or larger) under the null-hypothesis. We can then threshold this map with clusters, using a ‘cluster-wise’ \(p\)-value cutoff of 0.01 for example, and plot it again to see how this method affects type 1 and type 2 errors. The function below (threshold_RFT_cluster) takes three arguments: the statistics-map (our data variable), a minimum \(z\)-value, and a \(p\)-value cutoff which is used to threshold the clusters.
Below, we do this for a \(z\)-threshold of 3.1 (corresponding to a \(p\)-value of approx. 0.001) and a cluster \(p\)-value threshold of 0.05.
from niedu.utils.nii import rft_cluster_threshold
rft_cl_mask = rft_cluster_threshold(data, z_thresh=3.1, p_clust=0.01)
plot_sim_brain(data, mask=rft_cl_mask, title='RFT thresholding (cluster-based)')
From the above plots, you should see that cluster-thresholding can be a very sensitive way to threshold your data if you expect your effects to occur in relatively large clusters (and given that you’re able to estimate the smoothness of the data appropriately, something that is a topic of debate). As such, it is by far the most used MCC method in univariate fMRI research today (but this does not necessarily mean it’s the best way).
Non-parametric MCC#
In addition to the previously discussed MCC approaches (which are common in parametric group-level models), non-parametric analyses offer another approach. In this approach, the algorithm keeps track of the maximum statistic across permutations. This statistic can refer to the highest voxel-wise amplitude (for voxel-based), largest cluster size (for cluster-based, given some initial \(z\)-value cutoff), or even highest TFCE-transformed amplitude, across permutations.
If we, for example, want to perform a cluster-based non-parametric analyses, we can save the largest cluster size (given some initial \(z\)-value threshold) for each iteration. Then, across our (let’s say) 5000 permutations, we have acquired a distribution of maximum cluster sizes under the null hypothesis of no effect.
We actually did this for our simulated data: we kept track of the maximum cluster size across 1000 permutations given some initial \(z\)-value cutoff. We’ll plot such a non-parametric distribution below (for an arbitrary \(z\)-value cutoff of 3):
np_dist = np.load('clust_size_dist_data.npz')
zx, clust_sizes = np_dist['zx'], np_dist['dist']
z_cutoff = 3
z_idx = np.abs(zx - z_cutoff).argmin()
clust_size_dist = clust_sizes[:, z_idx]
plt.figure(figsize=(15, 5))
plt.title("Max. cluster size across 1000 permutations", fontsize=25)
plt.hist(clust_size_dist, bins=50)
plt.xlabel("Max. cluster size", fontsize=20)
plt.ylabel("Frequency", fontsize=20)
plt.grid()
plt.show()
With that information, we can calculate the non-parametric \(p\)-value of each of our observed clusters using the same type of formula as we used earlier:
(86)#\[\begin{align}
p_{\mathrm{cluster}} = \frac{\sum_{i=1}^{P}\mathbf{I}(\mathbf{\mathrm{max.\ cluster\ size}}^{\mathrm{perm}}_{i} \geq \mathrm{cluster\ size}^{\mathrm{obs}}) + 1}{P + 1}
\end{align}\]
The same logic holds for voxel-based (TFCE-transformed) amplitude, where you wouldn’t keep track of the maximum cluster size, but the maximum amplitude across permutations.
ToDo (ungraded/optional): Suppose I have found an effect within our simulated data with a cluster size of 231 "voxels" (using an initial $z$-value threshold of 3). Using the distribution of maximum cluster sizes above (i.e., the variable clust_size_dist), can you compute the associated cluster $p$-value? Store it in a variable named pval_clust_size.
""" Implement the (optional) ToDo here. """
# YOUR CODE HERE
raise NotImplementedError()
''' Tests the above (optional) ToDo. '''
np.testing.assert_almost_equal(pval_clust_size, 0.001998)
print("Well done!")
In our experience, non-parametric analyses (e.g., randomise in FSL) in combination with TFCE (also supported in FSL) is a very sensitive approach, allowing for voxel-wise inference while taking into account the “blobbiness” of effects!
Exercise on new data#
Suppose we repeat the cat-picture experiment which we described earlier. Based on the literature, we expect to find strong activation in a small group of voxels — known as the nucleus felix — which is about 29 “voxels” in volume, located in the middle of the brain (here: our 2D brain). Like our other example, we’ve measured a group-level (2D) statistics (\(z\)-values) map which represents the cat-against-baseline contrast.
We’ll load in and plot the new data below:
data2 = np.load('data_assignment.npy')
plot_sim_brain(data2, title='Simulated data assignment', vmin=-10, vmax=10)
ToDo/ToThink (1 point): Given that cluster-based RFT correction worked really well in our last example, should we use this technique again on this dataset, given our expectations of the true effect? Why (not)? Hint: actually apply the cluster-based RFT correction to the new data (you may assume that the new data has the same smoothness as the previous data).
# Apply cluster-based RFT
YOUR ANSWER HERE
Effect of different MCC strategies on real data#
We actually ran group-level analyses (using FLAME1-type mixed-effects) with different MCC methods on our run-level \(4\cdot \beta_{face} - \beta_{place} - \beta_{body} - \beta_{character} - \beta_{object}\) contrast from 12 subjects, which we download below:
import os
data_dir = os.path.join(os.path.expanduser("~"), 'NI-edu-data')
print("Downloading group-level FSL FEAT results (+- 133MB) ...")
!aws s3 sync --no-sign-request s3://openneuro.org/ds003965 {data_dir} --exclude "*" --include "derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1*/*"
print("\nDone!")
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Completed 7.7 MiB/~61.4 MiB (5.8 MiB/s) with ~214 file(s) remaining (calculating...)
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Completed 8.5 MiB/~61.4 MiB (5.9 MiB/s) with ~197 file(s) remaining (calculating...)
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Completed 8.7 MiB/~61.4 MiB (6.0 MiB/s) with ~195 file(s) remaining (calculating...)
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download: s3://openneuro.org/ds003965/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat/logs/feat3b_flame.e22347.70 to ../../../../../../NI-edu-data/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat/logs/feat3b_flame.e22347.70
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download: s3://openneuro.org/ds003965/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat/logs/feat3b_flame.e22347.68 to ../../../../../../NI-edu-data/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat/logs/feat3b_flame.e22347.68
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Completed 11.0 MiB/~61.4 MiB (6.6 MiB/s) with ~167 file(s) remaining (calculating...)
download: s3://openneuro.org/ds003965/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat/example_func.nii.gz to ../../../../../../NI-edu-data/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat/example_func.nii.gz
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Completed 11.5 MiB/~61.4 MiB (6.9 MiB/s) with ~166 file(s) remaining (calculating...)
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Completed 13.4 MiB/~61.4 MiB (7.9 MiB/s) with ~157 file(s) remaining (calculating...)
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Completed 13.4 MiB/~61.4 MiB (7.9 MiB/s) with ~156 file(s) remaining (calculating...)
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Completed 15.7 MiB/~61.4 MiB (9.0 MiB/s) with ~148 file(s) remaining (calculating...)
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Completed 15.7 MiB/~61.4 MiB (9.0 MiB/s) with ~147 file(s) remaining (calculating...)
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Completed 15.7 MiB/~61.4 MiB (8.8 MiB/s) with ~143 file(s) remaining (calculating...)
Completed 15.7 MiB/~61.4 MiB (8.8 MiB/s) with ~143 file(s) remaining (calculating...)
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Completed 15.9 MiB/~61.4 MiB (8.8 MiB/s) with ~143 file(s) remaining (calculating...)
download: s3://openneuro.org/ds003965/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat/logs/feat3b_flame.o22347.3 to ../../../../../../NI-edu-data/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat/logs/feat3b_flame.o22347.3
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download: s3://openneuro.org/ds003965/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat/logs/feat3b_flame.o22347.26 to ../../../../../../NI-edu-data/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat/logs/feat3b_flame.o22347.26
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download: s3://openneuro.org/ds003965/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat/logs/feat3b_flame.o22347.30 to ../../../../../../NI-edu-data/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat/logs/feat3b_flame.o22347.30
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Completed 16.7 MiB/~61.4 MiB (9.2 MiB/s) with ~139 file(s) remaining (calculating...)
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Completed 16.7 MiB/~61.4 MiB (9.0 MiB/s) with ~135 file(s) remaining (calculating...)
download: s3://openneuro.org/ds003965/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat/logs/feat3b_flame.o22347.35 to ../../../../../../NI-edu-data/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat/logs/feat3b_flame.o22347.35
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Completed 16.9 MiB/~61.4 MiB (9.0 MiB/s) with ~133 file(s) remaining (calculating...)
download: s3://openneuro.org/ds003965/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat/logs/feat3b_flame.o22347.40 to ../../../../../../NI-edu-data/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat/logs/feat3b_flame.o22347.40
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Completed 16.9 MiB/~61.4 MiB (8.9 MiB/s) with ~130 file(s) remaining (calculating...)
Completed 16.9 MiB/~61.4 MiB (8.9 MiB/s) with ~130 file(s) remaining (calculating...)
Completed 16.9 MiB/~61.4 MiB (8.9 MiB/s) with ~130 file(s) remaining (calculating...)
Completed 16.9 MiB/~61.4 MiB (8.9 MiB/s) with ~130 file(s) remaining (calculating...)
Completed 16.9 MiB/~61.4 MiB (8.8 MiB/s) with ~130 file(s) remaining (calculating...)
download: s3://openneuro.org/ds003965/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat/logs/feat3b_flame.o22347.4 to ../../../../../../NI-edu-data/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat/logs/feat3b_flame.o22347.4
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Completed 16.9 MiB/~61.4 MiB (8.8 MiB/s) with ~129 file(s) remaining (calculating...)
download: s3://openneuro.org/ds003965/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat/logs/feat3b_flame.o22347.45 to ../../../../../../NI-edu-data/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat/logs/feat3b_flame.o22347.45
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Completed 16.9 MiB/~61.4 MiB (8.8 MiB/s) with ~126 file(s) remaining (calculating...)
download: s3://openneuro.org/ds003965/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat/logs/feat3b_flame.o22347.43 to ../../../../../../NI-edu-data/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat/logs/feat3b_flame.o22347.43
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Completed 16.9 MiB/~61.4 MiB (8.7 MiB/s) with ~124 file(s) remaining (calculating...)
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Completed 16.9 MiB/~61.4 MiB (8.6 MiB/s) with ~121 file(s) remaining (calculating...)
download: s3://openneuro.org/ds003965/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat/logs/feat3b_flame.o22347.44 to ../../../../../../NI-edu-data/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat/logs/feat3b_flame.o22347.44
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Completed 17.2 MiB/~61.4 MiB (8.7 MiB/s) with ~120 file(s) remaining (calculating...)
download: s3://openneuro.org/ds003965/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat/filtered_func_data.nii.gz to ../../../../../../NI-edu-data/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat/filtered_func_data.nii.gz
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download: s3://openneuro.org/ds003965/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat/logs/feat3b_flame.o22347.52 to ../../../../../../NI-edu-data/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat/logs/feat3b_flame.o22347.52
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Completed 17.2 MiB/~61.4 MiB (8.7 MiB/s) with ~118 file(s) remaining (calculating...)
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Completed 17.2 MiB/~61.4 MiB (8.6 MiB/s) with ~115 file(s) remaining (calculating...)
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Completed 17.2 MiB/~61.4 MiB (8.6 MiB/s) with ~114 file(s) remaining (calculating...)
Completed 17.2 MiB/~61.4 MiB (8.6 MiB/s) with ~114 file(s) remaining (calculating...)
download: s3://openneuro.org/ds003965/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat/logs/feat3b_flame.o22347.55 to ../../../../../../NI-edu-data/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat/logs/feat3b_flame.o22347.55
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Completed 17.2 MiB/~61.4 MiB (8.4 MiB/s) with ~113 file(s) remaining (calculating...)
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Completed 17.2 MiB/~61.4 MiB (8.4 MiB/s) with ~110 file(s) remaining (calculating...)
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download: s3://openneuro.org/ds003965/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat/logs/feat3b_flame.o22347.48 to ../../../../../../NI-edu-data/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat/logs/feat3b_flame.o22347.48
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download: s3://openneuro.org/ds003965/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat/logs/feat3b_flame.o22347.5 to ../../../../../../NI-edu-data/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat/logs/feat3b_flame.o22347.5
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download: s3://openneuro.org/ds003965/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat/logs/feat3b_flame.o22347.59 to ../../../../../../NI-edu-data/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat/logs/feat3b_flame.o22347.59
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Completed 17.2 MiB/~61.4 MiB (8.3 MiB/s) with ~104 file(s) remaining (calculating...)
Completed 17.2 MiB/~61.4 MiB (8.3 MiB/s) with ~104 file(s) remaining (calculating...)
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Completed 17.2 MiB/~61.4 MiB (8.2 MiB/s) with ~104 file(s) remaining (calculating...)
Completed 17.2 MiB/~61.4 MiB (8.2 MiB/s) with ~104 file(s) remaining (calculating...)
download: s3://openneuro.org/ds003965/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat/logs/feat3b_flame.o22347.60 to ../../../../../../NI-edu-data/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat/logs/feat3b_flame.o22347.60
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Completed 17.2 MiB/~61.4 MiB (8.2 MiB/s) with ~103 file(s) remaining (calculating...)
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Completed 17.2 MiB/~61.4 MiB (8.2 MiB/s) with ~100 file(s) remaining (calculating...)
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download: s3://openneuro.org/ds003965/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat/logs/feat3b_flame.o22347.7 to ../../../../../../NI-edu-data/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat/logs/feat3b_flame.o22347.7
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Completed 17.2 MiB/~61.4 MiB (8.1 MiB/s) with ~96 file(s) remaining (calculating...)
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Completed 17.2 MiB/~61.4 MiB (8.1 MiB/s) with ~95 file(s) remaining (calculating...)
Completed 17.2 MiB/~61.4 MiB (8.1 MiB/s) with ~95 file(s) remaining (calculating...)
Completed 17.2 MiB/~61.4 MiB (8.1 MiB/s) with ~95 file(s) remaining (calculating...)
Completed 17.2 MiB/~61.4 MiB (8.1 MiB/s) with ~95 file(s) remaining (calculating...)
download: s3://openneuro.org/ds003965/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat/logs/feat3b_flame.o22347.73 to ../../../../../../NI-edu-data/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat/logs/feat3b_flame.o22347.73
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download: s3://openneuro.org/ds003965/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat/logs/feat3b_flame.o22347.72 to ../../../../../../NI-edu-data/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat/logs/feat3b_flame.o22347.72
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download: s3://openneuro.org/ds003965/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat/logs/feat3b_flame.o22347.75 to ../../../../../../NI-edu-data/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat/logs/feat3b_flame.o22347.75
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Completed 17.2 MiB/~61.4 MiB (7.9 MiB/s) with ~91 file(s) remaining (calculating...)
Completed 17.2 MiB/~61.4 MiB (7.9 MiB/s) with ~91 file(s) remaining (calculating...)
Completed 17.2 MiB/~61.4 MiB (7.9 MiB/s) with ~91 file(s) remaining (calculating...)
Completed 17.2 MiB/~61.4 MiB (7.9 MiB/s) with ~91 file(s) remaining (calculating...)
Completed 17.2 MiB/~61.4 MiB (7.9 MiB/s) with ~91 file(s) remaining (calculating...)
download: s3://openneuro.org/ds003965/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat/logs/feat3b_flame.o22347.74 to ../../../../../../NI-edu-data/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat/logs/feat3b_flame.o22347.74
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Completed 17.2 MiB/~61.4 MiB (7.9 MiB/s) with ~90 file(s) remaining (calculating...)
Completed 17.2 MiB/~61.4 MiB (7.9 MiB/s) with ~90 file(s) remaining (calculating...)
download: s3://openneuro.org/ds003965/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat/logs/feat3b_flame.o22347.82 to ../../../../../../NI-edu-data/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat/logs/feat3b_flame.o22347.82
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download: s3://openneuro.org/ds003965/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat/logs/feat3b_flame.o22347.67 to ../../../../../../NI-edu-data/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat/logs/feat3b_flame.o22347.67
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download: s3://openneuro.org/ds003965/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat/logs/feat3b_flame.o22347.8 to ../../../../../../NI-edu-data/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat/logs/feat3b_flame.o22347.8
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download: s3://openneuro.org/ds003965/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat/logs/feat3b_flame.o22347.79 to ../../../../../../NI-edu-data/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat/logs/feat3b_flame.o22347.79
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Completed 17.2 MiB/~61.4 MiB (7.8 MiB/s) with ~86 file(s) remaining (calculating...)
download: s3://openneuro.org/ds003965/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat/logs/feat3b_flame.o22347.78 to ../../../../../../NI-edu-data/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat/logs/feat3b_flame.o22347.78
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download: s3://openneuro.org/ds003965/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat/logs/feat3b_flame.o22347.76 to ../../../../../../NI-edu-data/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat/logs/feat3b_flame.o22347.76
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download: s3://openneuro.org/ds003965/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat/logs/feat3b_flame.o22347.80 to ../../../../../../NI-edu-data/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat/logs/feat3b_flame.o22347.80
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download: s3://openneuro.org/ds003965/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat/logs/feat3b_flame.o22347.77 to ../../../../../../NI-edu-data/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat/logs/feat3b_flame.o22347.77
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Completed 17.2 MiB/~61.4 MiB (7.8 MiB/s) with ~82 file(s) remaining (calculating...)
download: s3://openneuro.org/ds003965/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat/logs/feat3b_flame.o22347.81 to ../../../../../../NI-edu-data/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat/logs/feat3b_flame.o22347.81
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download: s3://openneuro.org/ds003965/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat/stats/res4d.nii.gz to ../../../../../../NI-edu-data/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat/stats/res4d.nii.gz
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Done!
from glob import glob
fsl_deriv_dir = os.path.join(data_dir, 'derivatives', 'fsl')
gl_paths = sorted(glob(os.path.join(fsl_deriv_dir, 'grouplevel_task-flocBLOCKED', '*')))
print('\n'.join(gl_paths))
/home/runner/NI-edu-data/derivatives/fsl/grouplevel_task-flocBLOCKED/contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat
As you can see, there are results for three different MCC strategies:
uncorrected (with \(p < 0.05\));
cluster-based (with \(z > 3.1\) and \(p_{\mathrm{cluster}} < 0.05\));
voxel-based RFT (with \(p_{\mathrm{voxel}} > 0.05\));
non-parametric (“randomise” with TFCE, non-parametric \(p < 0.05\))
Let’s take a look at the thresholded \(z\)-statistic maps for each of those analyses.
ToDo (ungraded): If not yet opened, open FSLeyes. Add the standard MNI brain as a background image (File → Add standard → select MNI152_T1_2mm_brain.nii.gz). Then, add the thresh_zstat1.nii.gz image from the contrast-faceGTother_method-FLAME1_thresh-uncorr05.gfeat/cope1.feat directory (File → Add from file). Change the colormap to "Red-Yellow".
You have seen this brain map before, and you should know by now that this brain map likely contains many false positives as it’s not corrected for multiple comparisons.
ToDo (ungraded): Add the thresh_zstat1.nii.gz file from the contrast-faceGTother_method-FLAME1_thresh-cluster.gfeat/cope1.feat directory (File → Add from file). Change to colormap to "Blue-Light blue".
Here, you see a much more modest effect, where only a couple of clusters (in the superior temporal gyrus and posterior cingulate cortex) survived.
ToDo (ungraded): Add the thresh_zstat1.nii.gz file from the contrast-faceGTother_method-FLAME1_thresh-voxel.gfeat/cope1.feat directory, and change the colormap to "Green".
If you don’t see any green voxels, that’s right! No voxel “survived” the relatively conservative voxel-based RFT tresholding!
ToDo (ungraded): Lastly, add the thresh_zstat1.nii.gz file from the contrast-faceGTother_method-randmoise_thresh-TFCE05.gfeat/cope1.feat directory, and change the colormap to "Blue".
This looks quite alright (in the sense that at least some voxels survive the MCC procedure)! Does this mean that we should always use cluster-based or non-parametric (TFCE-boosted) MCC? Not necessarily. Like always, this depends on your data, the effect you expect, and the conclusions that you want to draw from your results.
Tip!
Before handing in your notebooks, we recommend restarting your kernel (Kernel → Restart & Clear Ouput) and running all your cells again (manually, or by Cell → Run all). By running all your cells one by one (from "top" to "bottom" of the notebook), you may spot potential errors that are caused by accidentally overwriting your variables or running your cells out of order (e.g., defining the variable 'x' in cell 28 which you then use in cell 15).